Journal: bioRxiv
Article Title: A Genetic Method for Distinguishing Cryptic Pocillopora Species in French Polynesia without Sequencing
doi: 10.64898/2026.04.25.720756
Figure Lengend Snippet: Gel band patterns generated by each restriction enzyme digest, which identify species of Pocillopora found in French Polynesia: Pocillopora acuta (P.a.), Pocillopora meandrina (P.m.), Pocillopora tuahiniensis (P.t.), Pocillopora cf. effusa (P.e.)., Pocillopora grandis (P.g.), Pocillopora verrucosa (P.v.), Left to right: NlaIV digest of the MtORF amplicon for all six Pocillopora species; EcoRV-HF digest of the MtORF amplicon for all six Pocillopora species; BseYI/AciI digest of the MtORF amplicon for all six Pocillopora species; XhoI digest of the PocHistone amplicon for all six Pocillopora species. The P. grandis digests are two different samples, representative of the (left to right) 2 band vs. 3 band cut patterns.
Article Snippet: A total of eight candidate enzymes were identified for further testing in silico : AciI (New England Biolabs (NEB) #R0551S, MA, USA), BanI (NEB #R0118S), BceAI (NEB #R0623S), BseYI (NEB #R0635S), EcoRV-HF (NEB #R3195S), HgaI (NEB #R0154S), NlaIV (NEB #R0126S), and SacI-HF (NEB #R3156S), and XhoI (NEB Cat # R0146S).
Techniques: Generated, Amplification